Transmembrane Location of Retinal in Purple Membrane
نویسندگان
چکیده
منابع مشابه
The transverse location of the retinal chromophore in the purple membrane by diffusion-enhanced energy transfer.
We have used fluorescence energy transfer in the rapid-diffusion limit (RDL) to estimate the trans-membrane depth of retinal in the purple membrane (PM). Chelates of Tb(III) are excellent energy donors for the retinal chromophore of PM, having a maximum Ro value for Förster energy transfer of approximately 62 A (assuming a donor quantum yield of 1). Energy transfer rates were measured from the ...
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as follows: 500 ml 230 medium 100 reciprocating movements per min for 5 days at Low purple membrane production is observed under conditions of sufficient aeration: cells were grown in Erlenmeyer flasks containing only 90 ml medium under otherwise in the presence of mM nicotine yielded cells with ml medium) or (90 ml of bacteria-opsin, respectively (‘nicotine [7 ] Basal salt solution contained l...
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Halobacteria are extremely specialized organisms. They live exclusively in saturated solutions of common salt. The cell membrane of these bacteria exhibit insular regions which can be isolated by membrane fractionation. These regions consist of a lipid matrix containing bateriorhodopsin molecules in a hexagonal crystalline arrangement. Bacteriorhodopsin is a deep purple retinal-protein complex ...
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The decay rate of the Bacteriorhodopsin (BR) photocycle intermediate M412 and proton, the proton pump efficiency (H+/M412), the ratios of M412 to other intermediates and the rotational correlation time (tauc) in purple membrane (PM) fragments treated by the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) with different concentrations were studied. The r...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1987
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(87)83240-x